LOCUS       40924_27633        5321 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pLD601 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Tomizawa K, Takase A, Kanamoto H.
  TITLE     A system for expressing GFP protein using plants
  JOURNAL   Published Only in Database (2005)
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Tomizawa K, Takase A, Kanamoto H.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2005) Hirosuke Kanamoto, Research Institute of 
            Innovative Technology for the Earth, Plant research group; 9-2 
            Kizugawadai, Kizu-cho, Kyoto 619-0292, Japan 
            (E-mail:kanamoto@rite.or.jp, Tel:81-774-75-2307, Fax:81-774-75-2320)
REFERENCE   3  (bases 1 to 5321)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5321)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Published 
            Only in Database (2005)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-JAN-2005) Hirosuke Kanamoto, Research Institute of Innovative 
            Technology for the Earth, Plant research group"; volume: " 9-2 
            Kizugawadai, Kizu-cho, Kyoto 619-0292, Japan 
            (E-mail:kanamoto@rite.or.jp, Tel:81-774-75-2307, Fax"; pages: 
            "81-774-75-2320"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..986
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      2226..2338
                     /label=tobacco rrn promoter
                     /note="tobacco rrn promoter"
                     /regulatory_class="promoter"
     CDS             2380..3165
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     regulatory      3573..3703
                     /label=tobacco psbA promoter
                     /note="tobacco psbA promoter"
                     /regulatory_class="promoter"
     RBS             3707..3718
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             3725..4438
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     promoter        complement(4678..4696)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4706..4722)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4864..5319
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg
     2221 gccgctctag ttggatttgc tcccccgccg tcgttcaatg agaatggata agaggctcgt
     2281 gggattgacg tgagggggca gggatggcta tatttctggg agcgaactcc gggcgaattt
     2341 gaagcgcttg gatacagttg tagggaggga tccatggctc gtgaagcggt tatcgccgaa
     2401 gtatcaactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     2461 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
     2521 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     2581 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     2641 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     2701 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     2761 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     2821 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     2881 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     2941 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     3001 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     3061 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     3121 tttgtccact acgtgaaagg cgagatcact aaggtagttg gcaaataact gcaggatcct
     3181 ggcctagtct ataggaggtt ttgaaaagaa aggagcaata atcattttct tgttctatca
     3241 agagggtgct attgctcctt tctttttttc tttttattta tttactagta ttttacttac
     3301 atagactttt ttgtttacat tatagaaaaa gaaggagagg ttattttctt gcatttattc
     3361 atgattgagt attctatttt gattttgtat ttgtttaaaa ttgtagaaat agaacttgtt
     3421 tctcttcttg ctaatgttac tatatctttt tgattttttt tttccaaaaa aaaatcaaat
     3481 tttgacttct tcttatctct tatctttgaa tatctcttat ctttgaaata ataatatcat
     3541 tgaaataaga aagaagagct atattcgaag cttctacata caccttggtt gacacgagta
     3601 tataagtcat gttatactgt tgaataacaa gccttccatt ttctattttg atttgtagaa
     3661 aactagtgtg cttgggagtc cctgatgatt aaataaacca agatctaaag aggagaaatt
     3721 aagcatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
     3781 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
     3841 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
     3901 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa
     3961 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
     4021 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
     4081 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
     4141 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
     4201 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
     4261 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
     4321 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
     4381 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata
     4441 atctagagtg gctagcgaca tcgatggcga attcgccctt ccgaagcttg aaattcaatt
     4501 aaggaaataa attaaggaaa tacaaaaagg ggggtagtca tttgtatata actttgtatg
     4561 acttttctct tctatttttt tgtatttcct ccctttcctt ttctatttgt atttttttat
     4621 cattgcttcc attgaattac tagtcgacct cgaggggggg cccggtaccc aattcgccct
     4681 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     4741 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     4801 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     4861 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4921 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4981 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5041 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     5101 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5161 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5221 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5281 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta g
//
